A linear, low-copy P. berghei artificial chromosome (PbAC) vector can stably maintain large fragments of P. berghei genomic DNA. Using the PbAC vector, we have generated an arrayed library of P. berghei artificial chromosomes harbouring large P. berghei genomic DNA inserts that provide an off-the-shelf complementation resource with no additional modifications required prior to transfection (unpublished). Enhancing gene copy numbers through PbAC clones can reveal proteins interacting with antiplasmodial compounds by sensitising or desensitising the parasite. PbACs can be modified by recombinase-mediated engineering, e.g., to generate epitope-tagged genes for localisation and expression studies.