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Clone libraries

PlasmoGEM genomic DNA clone libraries

Genome-spanning, mapped clones provide stable starting material for recombineering malaria parasite vectors.

Arrayed Plasmodium genomic libraries were constructed using the pJAZZ vector backbone. pJazz is a phage N15-derived vector that was once available from Lucigen (Godiska et al., 2010). pJAZZ is a low copy, linear vector especially suitable for maintaining large inserts of AT-rich DNA. The 14 kb long backbone of the vector contains a number of genetic features necessary for its propagation in the TSA strain of Escherichia coli. These features include hairpin telomers, a telomerase gene, replication factor and origin, and a selectable kanamycin resistance marker.

The gDNA inserts are end-sequenced and mapped to their genome of origin. The arrayed library clones are the starting point for recombineering. Gene search results show all genes with overlapping library clones; choose the “lib clone” button to inspect available material.

We are currently making available library clones for Plasmodium berghei and Plasmodium chabaudi. More than 97% of the annotated gene models are covered (>50% of CDS) in P. berghei and >80% in P. chabaudi. The PbG libraries encompass 9,946 genomic clones, with insert sizes ranging from 4-20 kb and an average insert length of 8.7 kb.